4 5l freeze dryer Search Results


freezone 2 5 l benchtop freeze dryer  (Labconco)
99
Labconco freezone 2 5 l benchtop freeze dryer
Freezone 2 5 L Benchtop Freeze Dryer, supplied by Labconco, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n bis  (Chem Impex International)
95
Chem Impex International n bis
N Bis, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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free thyroxine  (Valiant Co Ltd)
93
Valiant Co Ltd free thyroxine
Free Thyroxine, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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freeze dryer modulyod 5l  (Thermo Fisher)
90
Thermo Fisher freeze dryer modulyod 5l
Freeze Dryer Modulyod 5l, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microcystin-lr (mclr  (Millipore)
90
Millipore microcystin-lr (mclr
(A) Asynchronous or mitotic HeLa cell lysates were either treated with free <t>microcystin-LR</t> or left untreated, followed by enrichment of PPPome using PIBs. The PIB eluates from triplicate experiments were digested into peptides, labeled with tandem mass tags (TMT), combined, fractionated and analyzed by LC-MS/MS. (B) Volcano plot of proteins specifically bound to PIBs in asynchronous or mitotic lysates identified using PIB-TMT strategy. The proteins highlighted in red significantly increased and those in blue significantly decreased in their binding to PIBs in mitotic HeLa cells (fold-change mitotic/asynchronous being > or < respectively, P< 0.05, n=3 independent biological replicates). (C) Proteins identified by PIB-TMT strategy as either asynchronous-specific or mitosis-specific PPP binding proteins were validated by immunoblotting. (D) Scatter plot of the log2 ratio of PIB binding between mitotic and asynchronous cells of proteins specifically bound to PIBs in PIB-TMT experiment compared to global changes in protein abundance to determine correlation between PIB binding and protein expression. n=3 independent biological replicates.
Microcystin Lr (Mclr, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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freezone 4 5 l benchtop freeze dry system  (Labconco)
86
Labconco freezone 4 5 l benchtop freeze dry system
(A) Asynchronous or mitotic HeLa cell lysates were either treated with free <t>microcystin-LR</t> or left untreated, followed by enrichment of PPPome using PIBs. The PIB eluates from triplicate experiments were digested into peptides, labeled with tandem mass tags (TMT), combined, fractionated and analyzed by LC-MS/MS. (B) Volcano plot of proteins specifically bound to PIBs in asynchronous or mitotic lysates identified using PIB-TMT strategy. The proteins highlighted in red significantly increased and those in blue significantly decreased in their binding to PIBs in mitotic HeLa cells (fold-change mitotic/asynchronous being > or < respectively, P< 0.05, n=3 independent biological replicates). (C) Proteins identified by PIB-TMT strategy as either asynchronous-specific or mitosis-specific PPP binding proteins were validated by immunoblotting. (D) Scatter plot of the log2 ratio of PIB binding between mitotic and asynchronous cells of proteins specifically bound to PIBs in PIB-TMT experiment compared to global changes in protein abundance to determine correlation between PIB binding and protein expression. n=3 independent biological replicates.
Freezone 4 5 L Benchtop Freeze Dry System, supplied by Labconco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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freezone 4 5 l freeze dry system  (Labconco)
86
Labconco freezone 4 5 l freeze dry system
(A) Asynchronous or mitotic HeLa cell lysates were either treated with free <t>microcystin-LR</t> or left untreated, followed by enrichment of PPPome using PIBs. The PIB eluates from triplicate experiments were digested into peptides, labeled with tandem mass tags (TMT), combined, fractionated and analyzed by LC-MS/MS. (B) Volcano plot of proteins specifically bound to PIBs in asynchronous or mitotic lysates identified using PIB-TMT strategy. The proteins highlighted in red significantly increased and those in blue significantly decreased in their binding to PIBs in mitotic HeLa cells (fold-change mitotic/asynchronous being > or < respectively, P< 0.05, n=3 independent biological replicates). (C) Proteins identified by PIB-TMT strategy as either asynchronous-specific or mitosis-specific PPP binding proteins were validated by immunoblotting. (D) Scatter plot of the log2 ratio of PIB binding between mitotic and asynchronous cells of proteins specifically bound to PIBs in PIB-TMT experiment compared to global changes in protein abundance to determine correlation between PIB binding and protein expression. n=3 independent biological replicates.
Freezone 4 5 L Freeze Dry System, supplied by Labconco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/freezone 4 5 l freeze dry system/product/Labconco
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freezone 4 5l freeze dry system  (Labconco)
86
Labconco freezone 4 5l freeze dry system
(A) Asynchronous or mitotic HeLa cell lysates were either treated with free <t>microcystin-LR</t> or left untreated, followed by enrichment of PPPome using PIBs. The PIB eluates from triplicate experiments were digested into peptides, labeled with tandem mass tags (TMT), combined, fractionated and analyzed by LC-MS/MS. (B) Volcano plot of proteins specifically bound to PIBs in asynchronous or mitotic lysates identified using PIB-TMT strategy. The proteins highlighted in red significantly increased and those in blue significantly decreased in their binding to PIBs in mitotic HeLa cells (fold-change mitotic/asynchronous being > or < respectively, P< 0.05, n=3 independent biological replicates). (C) Proteins identified by PIB-TMT strategy as either asynchronous-specific or mitosis-specific PPP binding proteins were validated by immunoblotting. (D) Scatter plot of the log2 ratio of PIB binding between mitotic and asynchronous cells of proteins specifically bound to PIBs in PIB-TMT experiment compared to global changes in protein abundance to determine correlation between PIB binding and protein expression. n=3 independent biological replicates.
Freezone 4 5l Freeze Dry System, supplied by Labconco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/freezone 4 5l freeze dry system/product/Labconco
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freeze drying system type lyph lock 4 5 l  (Labconco)
86
Labconco freeze drying system type lyph lock 4 5 l
(A) Asynchronous or mitotic HeLa cell lysates were either treated with free <t>microcystin-LR</t> or left untreated, followed by enrichment of PPPome using PIBs. The PIB eluates from triplicate experiments were digested into peptides, labeled with tandem mass tags (TMT), combined, fractionated and analyzed by LC-MS/MS. (B) Volcano plot of proteins specifically bound to PIBs in asynchronous or mitotic lysates identified using PIB-TMT strategy. The proteins highlighted in red significantly increased and those in blue significantly decreased in their binding to PIBs in mitotic HeLa cells (fold-change mitotic/asynchronous being > or < respectively, P< 0.05, n=3 independent biological replicates). (C) Proteins identified by PIB-TMT strategy as either asynchronous-specific or mitosis-specific PPP binding proteins were validated by immunoblotting. (D) Scatter plot of the log2 ratio of PIB binding between mitotic and asynchronous cells of proteins specifically bound to PIBs in PIB-TMT experiment compared to global changes in protein abundance to determine correlation between PIB binding and protein expression. n=3 independent biological replicates.
Freeze Drying System Type Lyph Lock 4 5 L, supplied by Labconco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l tyrosine  (Chem Impex International)
95
Chem Impex International l tyrosine
(A) Asynchronous or mitotic HeLa cell lysates were either treated with free <t>microcystin-LR</t> or left untreated, followed by enrichment of PPPome using PIBs. The PIB eluates from triplicate experiments were digested into peptides, labeled with tandem mass tags (TMT), combined, fractionated and analyzed by LC-MS/MS. (B) Volcano plot of proteins specifically bound to PIBs in asynchronous or mitotic lysates identified using PIB-TMT strategy. The proteins highlighted in red significantly increased and those in blue significantly decreased in their binding to PIBs in mitotic HeLa cells (fold-change mitotic/asynchronous being > or < respectively, P< 0.05, n=3 independent biological replicates). (C) Proteins identified by PIB-TMT strategy as either asynchronous-specific or mitosis-specific PPP binding proteins were validated by immunoblotting. (D) Scatter plot of the log2 ratio of PIB binding between mitotic and asynchronous cells of proteins specifically bound to PIBs in PIB-TMT experiment compared to global changes in protein abundance to determine correlation between PIB binding and protein expression. n=3 independent biological replicates.
L Tyrosine, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scanvac coolsafe 4.5l  (LaboGene Inc)
90
LaboGene Inc scanvac coolsafe 4.5l
(A) Asynchronous or mitotic HeLa cell lysates were either treated with free <t>microcystin-LR</t> or left untreated, followed by enrichment of PPPome using PIBs. The PIB eluates from triplicate experiments were digested into peptides, labeled with tandem mass tags (TMT), combined, fractionated and analyzed by LC-MS/MS. (B) Volcano plot of proteins specifically bound to PIBs in asynchronous or mitotic lysates identified using PIB-TMT strategy. The proteins highlighted in red significantly increased and those in blue significantly decreased in their binding to PIBs in mitotic HeLa cells (fold-change mitotic/asynchronous being > or < respectively, P< 0.05, n=3 independent biological replicates). (C) Proteins identified by PIB-TMT strategy as either asynchronous-specific or mitosis-specific PPP binding proteins were validated by immunoblotting. (D) Scatter plot of the log2 ratio of PIB binding between mitotic and asynchronous cells of proteins specifically bound to PIBs in PIB-TMT experiment compared to global changes in protein abundance to determine correlation between PIB binding and protein expression. n=3 independent biological replicates.
Scanvac Coolsafe 4.5l, supplied by LaboGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Asynchronous or mitotic HeLa cell lysates were either treated with free microcystin-LR or left untreated, followed by enrichment of PPPome using PIBs. The PIB eluates from triplicate experiments were digested into peptides, labeled with tandem mass tags (TMT), combined, fractionated and analyzed by LC-MS/MS. (B) Volcano plot of proteins specifically bound to PIBs in asynchronous or mitotic lysates identified using PIB-TMT strategy. The proteins highlighted in red significantly increased and those in blue significantly decreased in their binding to PIBs in mitotic HeLa cells (fold-change mitotic/asynchronous being > or < respectively, P< 0.05, n=3 independent biological replicates). (C) Proteins identified by PIB-TMT strategy as either asynchronous-specific or mitosis-specific PPP binding proteins were validated by immunoblotting. (D) Scatter plot of the log2 ratio of PIB binding between mitotic and asynchronous cells of proteins specifically bound to PIBs in PIB-TMT experiment compared to global changes in protein abundance to determine correlation between PIB binding and protein expression. n=3 independent biological replicates.

Journal: Science signaling

Article Title: Quantitative kinase and phosphatase profiling reveal that CDK1 phosphorylates PP2Ac to promote mitotic entry

doi: 10.1126/scisignal.aba7823

Figure Lengend Snippet: (A) Asynchronous or mitotic HeLa cell lysates were either treated with free microcystin-LR or left untreated, followed by enrichment of PPPome using PIBs. The PIB eluates from triplicate experiments were digested into peptides, labeled with tandem mass tags (TMT), combined, fractionated and analyzed by LC-MS/MS. (B) Volcano plot of proteins specifically bound to PIBs in asynchronous or mitotic lysates identified using PIB-TMT strategy. The proteins highlighted in red significantly increased and those in blue significantly decreased in their binding to PIBs in mitotic HeLa cells (fold-change mitotic/asynchronous being > or < respectively, P< 0.05, n=3 independent biological replicates). (C) Proteins identified by PIB-TMT strategy as either asynchronous-specific or mitosis-specific PPP binding proteins were validated by immunoblotting. (D) Scatter plot of the log2 ratio of PIB binding between mitotic and asynchronous cells of proteins specifically bound to PIBs in PIB-TMT experiment compared to global changes in protein abundance to determine correlation between PIB binding and protein expression. n=3 independent biological replicates.

Article Snippet: To determine the specific PIB binding by competition with free microcystin-LR (MCLR; Millipore), asynchronous or mitotic cell lysates were pre-incubated with 100 nM MCLR for 30 mins at 4°C before incubating with PIBs.

Techniques: Labeling, Liquid Chromatography with Mass Spectroscopy, Binding Assay, Western Blot, Expressing